2 replies to this topic

[franimaggie]

Hi all,

I am looking for suggestions on how to remove or disrupt the extracellular matrix of marine dinoflagellate cells without lysing or killing them.  Are there general enzymes available that would digest poly & oligosaccharides, or even glycoproteins?  I have also considered gentle agitation with glass beads to try and shear the glycocalyx off.  Has anyone ever tried this?

Also, I need a method to visualize the matrix in order to determine if disruption was successful.
Any help would be wonderful--

thanks in advance,

franchesca perez
Shannon Point Marine Center
Anacortes, WA

[Nacho Bravo]

Try hyaluronidase and syalidase, or choose any other depending on the chemical nature of your matrix.

[Damiano Vesentini]

I am extracting extracellular mucilage from filamentous fungi by washing the colony with cold water. it works, as electron microscopy has shown.