6 replies to this topic

[ikwana]

can sumbody help me, how to find antioxidant gene in garlic?
i try to search at NCBI, but there is a lot of gene about garlic come out..
i dono which one code for the antioxidant which is allicin.. i hv to order primer from it..

[bob1]

As far as I can see there is no gene for Allicin, it is produced by the enzyme Alliinase acting on Alliin which is a modified cysteine.  There are two entries on NCBI for Allicin production from bacteria and fungus respectively.

[ikwana]

bob1, on 10 August 2010 - 03:01 PM, said:

As far as I can see there is no gene for Allicin, it is produced by the enzyme Alliinase acting on Alliin which is a modified cysteine.  There are two entries on NCBI for Allicin production from bacteria and fungus respectively.

the production is in wut category? nucleotide or jurnal? how am i going to get the sequence of allinase production?  

[bob1]

http://www.ncbi.nlm....y/?term=ALLICIN Have a look at the Nucleotide link.

[ikwana]

bob1, on 11 August 2010 - 02:25 PM, said:

http://www.ncbi.nlm....y/?term=ALLICIN Have a look at the Nucleotide link.

yeaaa!! got the sequence! it codes for allinase rite?
but why the source of organism is fusarium?  
when i blast the sequence, it comes out the sinorhizobium, wuts their correlation?   
i hv another problem, when i click for pick primer, it says that "Primer specificity cannot be properly determined as sequences from the specified organism (Fusarium sp.) are not present in selected database: refseq_rna. "

should i go to protein sequence instead of nucleotide.. cuz i hv to do pcr for gene responsible codes for allinase? or should i search for allin? its kinda confuse

[bob1]

Those genes are ones from Fusarium, which is a fungus (sinorhizobium is a bacterium) and will not be the same as the one found in garlic, though there may well be some similarities.

Try http://www.ncbi.nlm....orgn:__txid4682 for the alliinase genes.  Look for complete cds.

[ikwana]

bob1, on 12 August 2010 - 12:48 PM, said:

Those genes are ones from Fusarium, which is a fungus (sinorhizobium is a bacterium) and will not be the same as the one found in garlic, though there may well be some similarities.

Try http://www.ncbi.nlm....orgn:__txid4682 for the alliinase genes.  Look for complete cds.

ok thx.. i saved dat sequnce.. but now i want to find sequence for alliin.. when i search allin at NCBI, it comes out the allin lyase.. from what i know, allin lyase is another name for alliinase rite?