
allium sativum (garlic) and its antioxidant
#1
Posted 10 August 2010 - 06:53 AM
i try to search at NCBI, but there is a lot of gene about garlic come out..
i dono which one code for the antioxidant which is allicin.. i hv to order primer from it..
#2
Posted 10 August 2010 - 03:01 PM
#3
Posted 11 August 2010 - 01:49 AM
As far as I can see there is no gene for Allicin, it is produced by the enzyme Alliinase acting on Alliin which is a modified cysteine. There are two entries on NCBI for Allicin production from bacteria and fungus respectively.
the production is in wut category? nucleotide or jurnal? how am i going to get the sequence of allinase production?

#4
Posted 11 August 2010 - 02:25 PM
#5
Posted 11 August 2010 - 10:24 PM
http://www.ncbi.nlm....y/?term=ALLICIN Have a look at the Nucleotide link.

but why the source of organism is fusarium?

when i blast the sequence, it comes out the sinorhizobium, wuts their correlation?

i hv another problem, when i click for pick primer, it says that "Primer specificity cannot be properly determined as sequences from the specified organism (Fusarium sp.) are not present in selected database: refseq_rna. "

should i go to protein sequence instead of nucleotide.. cuz i hv to do pcr for gene responsible codes for allinase? or should i search for allin? its kinda confuse

#6
Posted 12 August 2010 - 12:48 PM
Try http://www.ncbi.nlm....orgn:__txid4682 for the alliinase genes. Look for complete cds.
#7
Posted 14 August 2010 - 10:02 AM
Those genes are ones from Fusarium, which is a fungus (sinorhizobium is a bacterium) and will not be the same as the one found in garlic, though there may well be some similarities.
Try http://www.ncbi.nlm....orgn:__txid4682 for the alliinase genes. Look for complete cds.
ok thx.. i saved dat sequnce.. but now i want to find sequence for alliin.. when i search allin at NCBI, it comes out the allin lyase.. from what i know, allin lyase is another name for alliinase rite?