can sumbody help me, how to find antioxidant gene in garlic?
i try to search at NCBI, but there is a lot of gene about garlic come out..
i dono which one code for the antioxidant which is allicin.. i hv to order primer from it..
Submit your paper to J Biol Methods today!
6 replies to this topic
#2
bob1
-
- Global Moderators
-
- 5,904 posts
Thelymitra pulchella
425
Excellent
Excellent
Posted 10 August 2010 - 03:01 PM
As far as I can see there is no gene for Allicin, it is produced by the enzyme Alliinase acting on Alliin which is a modified cysteine. There are two entries on NCBI for Allicin production from bacteria and fungus respectively.
#3
ikwana
-
- Active Members
-
- 27 posts
member
0
Neutral
Neutral
Posted 11 August 2010 - 01:49 AM
As far as I can see there is no gene for Allicin, it is produced by the enzyme Alliinase acting on Alliin which is a modified cysteine. There are two entries on NCBI for Allicin production from bacteria and fungus respectively.
the production is in wut category? nucleotide or jurnal? how am i going to get the sequence of allinase production?
#4
bob1
-
- Global Moderators
-
- 5,904 posts
Thelymitra pulchella
425
Excellent
Excellent
Posted 11 August 2010 - 02:25 PM
http://www.ncbi.nlm....y/?term=ALLICIN Have a look at the Nucleotide link.
#5
ikwana
-
- Active Members
-
- 27 posts
member
0
Neutral
Neutral
Posted 11 August 2010 - 10:24 PM
http://www.ncbi.nlm....y/?term=ALLICIN Have a look at the Nucleotide link.
yeaaa!! got the sequence! it codes for allinase rite? but why the source of organism is fusarium?
when i blast the sequence, it comes out the sinorhizobium, wuts their correlation?
i hv another problem, when i click for pick primer, it says that "Primer specificity cannot be properly determined as sequences from the specified organism (Fusarium sp.) are not present in selected database: refseq_rna. "
should i go to protein sequence instead of nucleotide.. cuz i hv to do pcr for gene responsible codes for allinase? or should i search for allin? its kinda confuse
#6
bob1
-
- Global Moderators
-
- 5,904 posts
Thelymitra pulchella
425
Excellent
Excellent
Posted 12 August 2010 - 12:48 PM
Those genes are ones from Fusarium, which is a fungus (sinorhizobium is a bacterium) and will not be the same as the one found in garlic, though there may well be some similarities.
Try http://www.ncbi.nlm....orgn:__txid4682 for the alliinase genes. Look for complete cds.
Try http://www.ncbi.nlm....orgn:__txid4682 for the alliinase genes. Look for complete cds.
#7
ikwana
-
- Active Members
-
- 27 posts
member
0
Neutral
Neutral
Posted 14 August 2010 - 10:02 AM
Those genes are ones from Fusarium, which is a fungus (sinorhizobium is a bacterium) and will not be the same as the one found in garlic, though there may well be some similarities.
Try http://www.ncbi.nlm....orgn:__txid4682 for the alliinase genes. Look for complete cds.
ok thx.. i saved dat sequnce.. but now i want to find sequence for alliin.. when i search allin at NCBI, it comes out the allin lyase.. from what i know, allin lyase is another name for alliinase rite?
