I would like to chemicaly mutagenize plasmid DNA (double stranded). I cannot find any protocol for this, except for hydroxylamine mutagenesis.
I have 2 questions :
1. Is there any special reason, why is hydroxylamine most often used for plasmid DNA mutagenesis ?
2. If You have plasmid mutagenesis protocol for any other chemicals eg. EMS, please, send it to me.
Thank You in advance