I made 3% NuSieve gel
1* TBE buffer 50 ml
,and did electrophoresis twice.
I add 10 microliter of EtBr in the gel tank which is filled with 1* TBE buffer.
I put 20 microliters of each sample(w/4 microliters of gel loading buffer) and 20 microliters of 1kb ladder.
I did this for restriction mapping
But as you can see in the picture which I attached, the 1kb ladder did not move well (the far left lane without the number is for the ladder).
So I cannot tell the size.
Have you guys had the same kind of problems?
Doesn't the 1kb ladder move well in the 3% NuSieve gel?
I don't know what to do.
Edited by Eternal city, 09 August 2010 - 11:42 AM.