Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Is crosslinking necessary in ChIP?


  • Please log in to reply
1 reply to this topic

#1 Serendipitous

Serendipitous

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 08 August 2010 - 04:52 PM

Hi! Has anyone tried to ChIP either histones or sequence-specific transcription factors without prior crosslinking? How do the results compare?

#2 KPDE

KPDE

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 154 posts
0
Neutral

Posted 09 August 2010 - 09:58 AM

Hi! Has anyone tried to ChIP either histones or sequence-specific transcription factors without prior crosslinking? How do the results compare?


You should look up Native ChIP. As for experiences, a member of my old lab did native ChIP for some histone mods (don't remember which anymore) and got good results (not too much different from using crosslinked chromatin). This works because histones are so tightly bound to the DNA. TFs and other DNA bound proteins may not be bound tight enough to hang on through detergents and sonication and I don't know, personally, of anyone who has tried native ChIP for these types of proteins.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.