4 replies to this topic

[Bo Scales]

does anyone have a reasonably successful protocol for isolating dna from bones---bones that are highly compromised in integrity---i.e., really bad shape?  I've tried and am well seasoned in the traditional methods,e.g. sds pro K, salting out, PIC, silica/chaotropic , qiagen, zymo, ect.  Sometimes these things work and sometimes they don't.  Surely there is a protocol that works sucessfully most of the time.  Thank you

[AronD]

Bo,

Actually, the company I work for (Gentra) did some DNA purifications from ancient bone samples using the PUREGENE kit.  If you want, I can send you the paper on this (I think that it is still in press) for you to look over.  Please email me at adrake@gentra.com if you are interested and I can send it to you.

AronD

[sbidw]

get yourself this months Euro edition of Biotechniques ( a free really nice journal), you can also get  it online for free as well (do  a google search for biotechniques). In there they have an article on DNA extraction from bones from musem specimens. Ideal for what you want i reckon.

[sbidw]

opps forgot the citation:
BioTechniques 30:732-736 (2001)
Good luck.

[AronD]

sbidw had a great idea there!  You can also find it in the U.S. version of BioTechniques.  That citation is 22:402 (1997).