Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Ask for help w/ 3' RACE

  • Please log in to reply
No replies to this topic

#1 jiang21



  • Active Members
  • Pip
  • 5 posts

Posted 07 August 2010 - 08:23 PM

I have worked on 3' RACE for one gene for a couple of months. After RACE w/ Invitrogen kit, I tried several taqs from Invitrogen, Bioline, Strategen. I designed 6 gene specific primers (25-28 in length) for 3'. Because of many repeats @ 3' region, I have to design primers avoiding those regions - so expected products should be all > 1k bp.

I tried to optimize PCR conditions (AT, Mg, elonging time...). But each time I ended up w/ nothing (or primer dimers) or small mismatched bands (sequenced). Pretty frustrated!!! (Another gene 3' RACE worked so I think the RACE reation is fine. )

Would anyone please give me any suggestions or what kind of Taq you used or PCR conditions?? Thanks a lot!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.