primer sequence problem
Posted 07 August 2010 - 12:12 PM
what dies (T/C) mean?
should i order two primer and try both or what???
Posted 07 August 2010 - 02:15 PM
Posted 08 August 2010 - 04:46 AM
I would order that as "CCAAGGAYGGCAGCAGGCGCGAAA". As I understand it, most primer synthesis companies will then provide you with a mixture that is ~50% "CCAAGGATGGCAGCAGGCGCGAAA" and ~50% "CCAAGGACGGCAGCAGGCGCGAAA" -- but call the company to be sure. Why do you need the degeneracy?
Thank u v ery much for reply.
this primer sequence appear in a published paper that we do the same PCR protocol