Hello,
I am having some trouble purifying a DNA binding protein. It seems to be bound by some very strongly interacting nucleic acids, from the cell lysate, as suggested by the very high absorption at 260 nm on the UV.
Could you suggest any protocol(s) which will allow for the removal of such nucleic acids?
Thanks in advance,
Alex.
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3 replies to this topic
#2
HomeBrew
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Posted 07 August 2010 - 07:48 AM
Treatment with DNAse?
#3
aberibisky
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Posted 07 August 2010 - 08:33 AM
HomeBrew, on 07 August 2010 - 07:48 AM, said:
Treatment with DNAse?
Thanks for the suggestion, already tried adding some, did not work. Next time I'll add a bit more...
#4
hobglobin
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Posted 07 August 2010 - 10:16 AM
perhaps you should try to use an exonuclease (the "normal" DNAse is an endonuclease).
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
