Why there are so many plasmid in Chaperone competent cell set?
Posted 06 August 2010 - 05:50 PM
Link to product manual
Posted 08 August 2010 - 11:21 AM
Hello. I faced problem in expressing soluble protein in BL21. I would like to try out the expression with co-express chaperone. However, when going through TAKARA Chaperone Competent Cell BL21 series, I found out that there are 5 types of plasmid. When looking into detail, I think only two types of plasmid (pG-KJE8 and pTf16) are sufficient for experiment using the kit, because the design of different promoters in plasmid means we can induce chaperone expression by using different inducer. Does anybody have idea what's the role of the remaining three plasmids (pGRO7, pGKJE7 and pG-Tf2)? Do we really need them?
[url="[url]http://catalog.takara-bio.co.jp/en/PDFFiles/9120-25_e.pdf"]Link[/url] to product manual[/url]