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lambda phage library


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#1 proteinz

proteinz

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Posted 06 August 2010 - 08:01 AM

hi all,
I am starting a project which will require the construction of a lambda phage library from gDNA. I have researched the topic and most people in similar circumstances use ZAP express lambda as their vector. I have the protocol from Stratagene and am attempting to get through it but it is really unclear and as a newcomer in the area it is really daunting. Has anyone a printed protocol of how it is done in their laboratory? Or know any resource that may help?
thanks in advance

#2 proteinz

proteinz

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Posted 08 August 2010 - 11:53 PM

hi all,
I am starting a project which will require the construction of a lambda phage library from gDNA. I have researched the topic and most people in similar circumstances use ZAP express lambda as their vector. I have the protocol from Stratagene and am attempting to get through it but it is really unclear and as a newcomer in the area it is really daunting. Has anyone a printed protocol of how it is done in their laboratory? Or know any resource that may help?
thanks in advance

Anyone?




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