I make buffer Tris 20mM and EDTA 1mM with pH 8.0. I adjust the pH after dissolving both compunds. I notice that after putting the EDTA there is drop in the pH. Is it ok????
or I have to adjust the pH before adding the EDTA?????
REGARDS
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mdfenko
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Posted 05 August 2010 - 11:16 AM
adjust after adding edta (before adjusting to final volume) or make a stock of edta at pH 8 and dilute that into your adjusted buffer and you won't have to adjust again.
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