Finding bisulfite PCR primer sequence
Posted 05 August 2010 - 07:23 AM
I am having difficulty finding where my bisulfite converted primers are located on the Human iNOS gene on Chromosome 17.
My forward primer is: AATGAGAGTTGTTGTTGGGAAGTGTTT
My reverse primer is: CCACCAAACCCAACCAAACT
I really not sure where to start. I have tried using methBLAST, but have been unable to locate them.
If anyone can point me in the right direction, it would be greatly appreciated.
Posted 05 August 2010 - 01:34 PM
Posted 06 August 2010 - 12:25 PM
What am I doing wrong?
Posted 06 August 2010 - 01:14 PM