I am looking for a way to grow E. coli on plates that contain pH indicator, in such a way that I can detect colonies that cause a drop in pH in the surrounding media. The more sensitive, the better. Any sugestions?
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pH indicating plates
Started by shalomg, Mar 11 2002 01:57 PM
2 replies to this topic
#2
Vageesh
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Posted 18 April 2002 - 09:15 PM
Contact me at vageesh@iihr.kar.nic.in; We shall discuss; Even I am also interested in pH based microbial screening.
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hennry
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Posted 26 May 2003 - 09:18 AM
Hi,
as far as i know you can use nearly every pH-indicatorsubstance as long as its concentration is in a more or less non-toxic range, let's say 0,001.0,005%(w/v) as far as i know. The choice of indicator is then a function of your acid or base turnover.
Regards,
Henry
as far as i know you can use nearly every pH-indicatorsubstance as long as its concentration is in a more or less non-toxic range, let's say 0,001.0,005%(w/v) as far as i know. The choice of indicator is then a function of your acid or base turnover.
Regards,
Henry
