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pH indicating plates


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2 replies to this topic

#1 shalomg

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Posted 11 March 2002 - 01:57 PM

I am looking for a way to grow E. coli on plates that contain  pH indicator, in such a way that I can detect colonies that cause a drop in pH in the surrounding media.  The more sensitive, the better.  Any sugestions?

#2 Vageesh

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Posted 18 April 2002 - 09:15 PM

Contact me at vageesh@iihr.kar.nic.in; We shall discuss; Even I am also interested in pH based microbial screening.

#3 hennry

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Posted 26 May 2003 - 09:18 AM

Hi,
as far as i know you can use nearly every pH-indicatorsubstance as long as its concentration is in a more or less non-toxic range, let's say 0,001.0,005%(w/v) as far as i know. The choice of indicator is then a function of your acid or base turnover.
Regards,
Henry




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