Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

standard curve method for Q-PCR


  • Please log in to reply
3 replies to this topic

#1 cellculturenovis

cellculturenovis

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 05 August 2010 - 05:50 AM

Hi everyone I am a bit confused about some of the methods used to analyse Q-PCR data. I have read a bit about primer efficiencies, do you need the primer efficiencies of your target and reference genes to be similar if you are quantifying using the standard curve method?

#2 ElHo

ElHo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 90 posts
1
Neutral

Posted 05 August 2010 - 05:55 AM

As far as I know, no. Only if you use deltadeltaCT. And even then itīs not an absolute prerequisite as you can use the method developped by Pfaffl.

#3 cellculturenovis

cellculturenovis

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 05 August 2010 - 05:56 AM

Is the standard curve method as good as the CT method?

#4 ElHo

ElHo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 90 posts
1
Neutral

Posted 05 August 2010 - 06:26 AM

Quantification experiments by running standard curves in parallel are somewhat easier to design and fewer prerequisites must be met. But you also have higher material consumption.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.