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abnormal nuclear morphology after treating shRNA


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#1 iamdhan

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Posted 04 August 2010 - 11:45 PM

Hi,

After transfection with gene-of-my-interest-specific shRNA, there was a significant change in nuclear morphology.

It seems like it has something to do with maintaining normal nuclear structure, but I am not sure where to go from here with this.

I don't even know what to call this phenomenon in English. Therefore googling is not possible at the moment.

I know it is silly to show just one picture and ask other's opinion. Any short, rude, or half-minded reply might be a great help to me.

The confocal image is attached. Nucleus are colored in red and my gene is not in the frame cuz it was completely silenced.

I have checked control pool is perfectly normal.


What do you think? Why do nucleus have such a giant hole in them? It doesn't look like speckle.

Thanks!

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Edited by iamdhan, 05 August 2010 - 03:33 AM.


#2 bob1

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Posted 05 August 2010 - 01:58 PM

Looks like it might be the nucleoli beeing altered. Otherwise it is some sort of vacuole.

#3 iamdhan

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Posted 08 August 2010 - 07:00 PM

Thanks for your comment, bob1! I will search for more information according to them. :)




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