2 replies to this topic

[iamdhan]

Hi,

After transfection with gene-of-my-interest-specific shRNA, there was a significant change in nuclear morphology.

It seems like it has something to do with maintaining normal nuclear structure, but I am not sure where to go from here with this.

I don't even know what to call this phenomenon in English. Therefore googling is not possible at the moment.

I know it is silly to show just one picture and ask other's opinion. Any short, rude, or half-minded reply might be a great help to me.

The confocal image is attached. Nucleus are colored in red and my gene is not in the frame cuz it was completely silenced.

I have checked control pool is perfectly normal.


What do you think? Why do nucleus have such a giant hole in them? It doesn't look like speckle.

Thanks!

Attached Thumbnails

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Edited by iamdhan, 05 August 2010 - 03:33 AM.

[bob1]

Looks like it might be the nucleoli beeing altered.  Otherwise it is some sort of vacuole.

[iamdhan]

Thanks for your comment, bob1! I will search for more information according to them.