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PCR -No band formation


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#1 aks

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Posted 04 August 2010 - 02:36 PM

Hi,
I used the PV92 Informatics Kit from BIO-RAD to amplify the samples in the PCR. I followed the protocol as mentioned in the manual. After i ran the samples in the agarose gel, I could see only bands from the Molecular Mass Ladder(MMR), and migration of loading dye from the other wells. Is there any way I can check till what stage the protocol works and any idea as to why it is not working?
I have attached a picture of the gel after it was stained. The right most well was loaded with MMR (the one that has bands)
Thanks in advance for your help !!!IMG_0411.JPG

Edited by aks, 04 August 2010 - 02:53 PM.


#2 bob1

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Posted 04 August 2010 - 05:55 PM

See my other post in the mol biol sub-forum.... you need to start by saying whay you think might have gone wrong...




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