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strange glycerol stocks


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#1 antonio

antonio

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Posted 30 April 2002 - 04:56 PM

Hello,
I've had this wierd problem with some -80C glycerol stocks of XL-1 Blue cells with a pET28 vector + insert; when I streak the cells (kan-LB-agar) they form colonies and when I innoculate liquid cultures they grow to OD600 ~0.5-0.6 but then stop growing. I need to make minipreps, so I'd like an OD600 of 1.0. Has this ever happened to anyone, and if so what can be done?
Thanks,
Antonio  




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