I count cells without centrifuging and resuspending them but my supervisor insists on centrifuging and resuspending cells before counting them.
i dont understand does it make any difference because we are counting only the live cells and not the dead cells and debri..??
I have tried doing bothways at the same time there was a neglible difference. does any body else think my supervisor is right..?? if so is there a scientific reason behind this or is it just one of those "it's in the protocol" thing.
thanks in advance
Edited by bioforum, 04 August 2010 - 07:41 AM.