Posted 03 August 2010 - 07:00 AM
i am doing this gene isolation technique for the first time , here i need to perform the 3' race and 5' race in order to get the ends , i am having a doubt that the 3' end can be obtained with the oligo dt primers (anchored )only or the requirement of kit is a must.
Posted 05 August 2010 - 03:01 AM
dT primer: 5'- GCTGTCAACGATACGCTACGTAACGGCATGACAGTG(T)24 - 3'
3' RACE primer: 5'- GCTGTCAACGATACGCTACGTAACG - 3'
But the 5' RACE needs a ligation of special oligo and decaping of mRNA, so you maybe need the kit anyway.
Edited by Trof, 05 August 2010 - 03:03 AM.
I never trust anything that can't be doubted.