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primary pituitary culture


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#1 Kikki

Kikki

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Posted 03 August 2010 - 06:46 AM

Hi,
I'm going to start cultering rat primary anterior pituitary cells in the near future therefore I've been searching for some good culture medium for my cells.
Does anyone have an experience with the primary pituitary culture? Medium 199 with Earle’s salts, L-glutamine, sodium bicarbonate, HEPES supplemented with 10% fetal bovine serum would be enough for the culture ...?
Thanks for any help:)




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