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Protein A Sandwich ELISA

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#1 Mucinman



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Posted 03 August 2010 - 06:24 AM

Im trying to use a Protein A Coated ELISA plate for a sandwich ELISA. After I coat my rabbit capture antibody can I block the remaining Protein A sites with FBS?

#2 sgt4boston



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Posted 13 August 2010 - 11:48 AM

I saw no one was jumping in to respond. I would block the unreacted sites with a known mass/concentration of non-specific IgG. This would be necessary if the protein A reacts with conjugate or secondary ab or label.

If you use serum you do not know exactly the mass of non-specific IgG you are adding. This may not be a problem...but if you have access to some bulk IgG I would use that.

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