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MES buffer turned yellow


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5 replies to this topic

#1 katenkak

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Posted 03 August 2010 - 12:44 AM

Hi all!
Does anyone knows if I can still use NuPAGEŽ MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!

#2 mdfenko

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Posted 03 August 2010 - 07:00 AM

try it on unimportant samples.

but, in general, when in doubt, throw it out.
talent does what it can
genius does what it must
i do what i get paid to do

#3 purdue_biomed

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Posted 03 August 2010 - 07:08 AM

I did a western blot a few weeks ago with NuPage MES that turned yellow, and I had no issues. Invitrogen also said it shouldn't cause any problems...light exposure in the lab can also cause it to turn yellow over time.

#4 katenkak

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Posted 03 August 2010 - 07:35 AM

I did a western blot a few weeks ago with NuPage MES that turned yellow, and I had no issues. Invitrogen also said it shouldn't cause any problems...light exposure in the lab can also cause it to turn yellow over time.


Thanks a lot! NuPage buffers are rather expensive to through away when in doubt. :rolleyes:

#5 Prep!

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Posted 03 August 2010 - 09:37 PM

yeah u can always give it a shot before deciding against it but i wud also second wat md says... if no other optins available.. and u in doubt never use it!!! (tat is wat he meant by throwing away!!! i guess :D )

and yeah if things are expensive take better care!!! :P
good luck!!
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#6 Adrian K

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Posted 07 September 2010 - 07:55 AM

Hi all!
Does anyone knows if I can still use NuPAGE® MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!


LOL... you had it turned yellow...

I just got mine and is already in yellow.... duh....

Yes, I agree that this buffer is not cheap.

Edited by adrian kohsf, 07 September 2010 - 08:17 AM.

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