Hi all!
Does anyone knows if I can still use NuPAGEŽ MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!
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#2
mdfenko
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Posted 03 August 2010 - 07:00 AM
try it on unimportant samples.
but, in general, when in doubt, throw it out.
but, in general, when in doubt, throw it out.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
purdue_biomed
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Posted 03 August 2010 - 07:08 AM
I did a western blot a few weeks ago with NuPage MES that turned yellow, and I had no issues. Invitrogen also said it shouldn't cause any problems...light exposure in the lab can also cause it to turn yellow over time.
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katenkak
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Posted 03 August 2010 - 07:35 AM
purdue_biomed, on 03 August 2010 - 07:08 AM, said:
I did a western blot a few weeks ago with NuPage MES that turned yellow, and I had no issues. Invitrogen also said it shouldn't cause any problems...light exposure in the lab can also cause it to turn yellow over time.
Thanks a lot! NuPage buffers are rather expensive to through away when in doubt.
#5
Prep!
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Posted 03 August 2010 - 09:37 PM
yeah u can always give it a shot before deciding against it but i wud also second wat md says... if no other optins available.. and u in doubt never use it!!! (tat is wat he meant by throwing away!!! i guess 
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and yeah if things are expensive take better care!!!
good luck!!
)and yeah if things are expensive take better care!!!
good luck!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!
Cheers!!!
#6
Adrian K
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Posted 07 September 2010 - 07:55 AM
katenkak, on 03 August 2010 - 12:44 AM, said:
Hi all!
Does anyone knows if I can still use NuPAGE® MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!
Does anyone knows if I can still use NuPAGE® MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!
LOL... you had it turned yellow...
I just got mine and is already in yellow.... duh....
Yes, I agree that this buffer is not cheap.
Edited by adrian kohsf, 07 September 2010 - 08:17 AM.
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
