MES buffer turned yellow
Posted 03 August 2010 - 12:44 AM
Does anyone knows if I can still use NuPAGEŽ MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!
Posted 03 August 2010 - 07:00 AM
but, in general, when in doubt, throw it out.
genius does what it must
i do what i get paid to do
Posted 03 August 2010 - 07:08 AM
Posted 03 August 2010 - 07:35 AM
I did a western blot a few weeks ago with NuPage MES that turned yellow, and I had no issues. Invitrogen also said it shouldn't cause any problems...light exposure in the lab can also cause it to turn yellow over time.
Thanks a lot! NuPage buffers are rather expensive to through away when in doubt.
Posted 03 August 2010 - 09:37 PM
and yeah if things are expensive take better care!!!
Posted 07 September 2010 - 07:55 AM
Does anyone knows if I can still use NuPAGE® MES SDS Running Buffer (20x) after it has turned yellow? It was transparent, but aapparently after a heat wave (was rather hot in the lab) it became yellow. Thank you for answer!
LOL... you had it turned yellow...
I just got mine and is already in yellow.... duh....
Yes, I agree that this buffer is not cheap.
Edited by adrian kohsf, 07 September 2010 - 08:17 AM.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434