HI FRIENDS
I AM A PLANT MOLECULAR BIOLOGY STUDENT. I HAVE PREPARED THE GENE SPECIFIC PRIMERS. MY PROBELEM IS THAT I GOT AMPLIFICATION WITH cDNA BUT UNABLE TO GOT FROM THE GENOMIC DNA. i AM UNABLE TO TROUBLESHOOT THIS.
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Adrian K
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Posted 03 August 2010 - 08:08 AM
PRATIPAL, on 02 August 2010 - 09:15 PM, said:
HI FRIENDS
I AM A PLANT MOLECULAR BIOLOGY STUDENT. I HAVE PREPARED THE GENE SPECIFIC PRIMERS. MY PROBELEM IS THAT I GOT AMPLIFICATION WITH cDNA BUT UNABLE TO GOT FROM THE GENOMIC DNA. i AM UNABLE TO TROUBLESHOOT THIS.
I AM A PLANT MOLECULAR BIOLOGY STUDENT. I HAVE PREPARED THE GENE SPECIFIC PRIMERS. MY PROBELEM IS THAT I GOT AMPLIFICATION WITH cDNA BUT UNABLE TO GOT FROM THE GENOMIC DNA. i AM UNABLE TO TROUBLESHOOT THIS.
Did your primer spans the intron region, and only amplified the spliced exon?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
