Fixing cells for flow cytometry
Posted 02 August 2010 - 06:59 PM
Posted 03 August 2010 - 01:55 AM
You could try EtOH fixation? (depending on why you want to fix cells). I produced a monoclonal antibody to a transcription factor and found it only worked (well) when I EtOH fixed the cells (and not PFA). I fixed my cells in 70% EtOH (in PBS) ON at 4degC. Note: I stained cells the following day. Hope this helps!
Posted 11 August 2010 - 08:26 AM
30 mL methanol free formadehyde to 270 mL of 0.1% bovine serum albumin solution in phosphate buffered saline.
pH to 7.4+/-0.1
store at 4 degrees C in a dark bottle.