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[pDNA]

Dear people,

     does anyone know where the neomycin phosotransferase (nptII, the common kanamycin resistance found in many cloning vectors) is localized in E. coli?
Is it a cytoplasmic protein or is located in the periplasmic space? Does anyone know relevant literature on this topic?

Many thanks for your help!

Best regards,
p

[pDNA]

okay, i will answer my question

According to brenda its secreted into the periplasmic space:

My link

Regards,
p