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Localization of kanamycin resistance protein


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#1 pDNA

pDNA

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Posted 02 August 2010 - 10:53 AM

Dear people,

does anyone know where the neomycin phosotransferase (nptII, the common kanamycin resistance found in many cloning vectors) is localized in E. coli?
Is it a cytoplasmic protein or is located in the periplasmic space? Does anyone know relevant literature on this topic?

Many thanks for your help!

Best regards,
p

#2 pDNA

pDNA

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Posted 03 August 2010 - 10:49 AM

okay, i will answer my question :)

According to brenda its secreted into the periplasmic space:

=Escherichia+coli&show_tm=0"]My link

Regards,
p




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