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buffer storage


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3 replies to this topic

#1 josephine

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Posted 02 August 2010 - 02:37 AM

How long would you store the following buffer (4 C):

60 mM Tris
2 mM EDTA
2 mM EGTA

pH 8,1 ?

I still have quite a lot in the cold room and I don't know if it is of any use after one year.

Thanks for help!

j

#2 phage434

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Posted 02 August 2010 - 04:59 AM

Hard to believe anything is growing in it. It's probably ok.

#3 josephine

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Posted 02 August 2010 - 05:18 AM

Hard to believe anything is growing in it. It's probably ok.



I don't think anything is growing there either, at least macroscopically it looks clean.

What about other issues of stability etc?

#4 mdfenko

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Posted 02 August 2010 - 06:27 AM

all three components are stable. you can check the pH to ensure that there is no significant variation but make sure you check at the same temperature that you originally adjusted.
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