mycoplasma false positive
Posted 01 August 2010 - 10:59 PM
We use the Lonza MycoAlert test kit which works on luminescence to test for mycoplasma in our lab. We recently had 2 cultures that looked contaminated and we took some of the supernatant and plated it onto Agar plates and also placed it into TSB to grow overnight. The plates and TSB cultures looked like a standard bacterial contamination the next morning. We took some of the supernatant of the media and tested it for mycoplasma using the Lonza bioluminescence kit. It has come back as being positive for mycoplasma. The media was frozen before doing the myco testing. My question is this, can some other bugs give false positive for mycoplasma when using the luminescence kits?
We have had bacterial contamination from time to time but have never had a mycoplasma infection before so this is very worrying.
Any help would be greatly appreciated!
Posted 02 August 2010 - 01:24 PM
Most mycoplasma detection kits of the sort you are using are not specific enough to tell you if you have mycoplasma!
Posted 02 August 2010 - 02:55 PM