Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Enzymatic shear vs sonication

  • Please log in to reply
1 reply to this topic

#1 sci newbie

sci newbie


  • Members
  • Pip
  • 2 posts

Posted 01 August 2010 - 05:58 AM


I'm new to ChIP and have only tried enzymatic shearing so far (from activemotif). I was wondering if anyone has tried both methods and can give a take on them.
Using the enzymatic shearing kit, 200ul sheared DNA per IP and resuspending the extracted DNA in 100ul water, the positive control Ct was 28 (I used 5ul per PCR reaction). I'm guessing sonication might give me a better yield?

#2 Mighty Mouse

Mighty Mouse


  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 124 posts

Posted 01 August 2010 - 06:53 PM

I've never used both, just sonication. But from the folks I've talked to that have used enzymatic shearing, they say you get much smaller fragment sizes than in sonication, even with very brief enzyme exposure. So I guess if you know exactly what you're looking for then enzymatic shearing would be fine. If you want a larger "target" then sonication might be better...just a thought

We are all artists...painting with experience on the canvas of life

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.