I'm centrifuging them at 1500 x G for 15 minutes and washing with saline. Having a hard time getting all of the buffy coat. I think I ended up lysing the red blood cells as well. Had a slight conical shaped red film developing at the top of the sample against the side of the test tube (was this lysed cells?). Thanks, any tips would be appreciated.
Also: blood was collected in EDTA tubes.
Edited by Gradstudent78, 31 July 2010 - 03:52 PM.