I'm becoming familiar with the BCA assay. I have tissue extract from 30 animals of which I need to quantify the individual protein content. Diluting each of these extracts to a proper concentration takes quite some time, so by the time the last one has been diluted the first samples has been thawed for like 20 minutes. Should I be worried about protein degradation during that process? I have no feeling for how much a concern proteases are in general. I might just be paranoid from spending too many hours in the RNA lab, where RNAses are quick to punish inconsiderate behavior
Edited by Jens Madsen, 31 July 2010 - 12:37 AM.