Hi all, in my lab I usually do overnight transfers for my gel, and the next morning when I take out the membrane and stains it with Ponceau red, i notices dark red spots appearing on the membrane (which almost looked like burned marks) but it washes out eventually using Tris saline and Tween solution. I am new to western so i'm not sure if this is normal or something wrong with my Ponceau/transfer/or other. Any advice would be appreciated.
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2 replies to this topic
#2
Gerard
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Posted 31 July 2010 - 06:51 AM
Itīs normal, Ponceau red washes away.
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#3
bob1
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Posted 01 August 2010 - 02:12 PM
It could be ponceau crystals, try filtering your solution.
