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Ponceau Red Staining Problem


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#1 pizzapop

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Posted 30 July 2010 - 06:59 AM

Hi all, in my lab I usually do overnight transfers for my gel, and the next morning when I take out the membrane and stains it with Ponceau red, i notices dark red spots appearing on the membrane (which almost looked like burned marks) but it washes out eventually using Tris saline and Tween solution. I am new to western so i'm not sure if this is normal or something wrong with my Ponceau/transfer/or other. Any advice would be appreciated.

#2 Gerard

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Posted 31 July 2010 - 06:51 AM

Itīs normal, Ponceau red washes away.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 bob1

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Posted 01 August 2010 - 02:12 PM

It could be ponceau crystals, try filtering your solution.




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