Hi all,
I would really appreciate some suggestions of internal controls I can use in my PCR using genomic DNA as a template.
I am working with human blood or marrow samples looking for chromosomal rearrangements. Is it possible to quantify rearrangement wrto a some sort of housekeeping gene when working with gNDA?
Thanks very much
internal control for genomic DNA
Started by labscientist, Jul 29 2010 10:32 PM
1 reply to this topic
#1
Posted 29 July 2010 - 10:32 PM
#2
Posted 30 July 2010 - 09:25 AM
For the expensive solutions, ABI sells IPC kits if you don't want to make them.
https://products.app...e2&catID=602714
Or you can use the Quantifiler kit that has everything in it
https://products.app...e2&catID=601641
If you'd rather make your own IPC, just browse the literature. There are plenty published.
Can't respond to the second question, sorry.
https://products.app...e2&catID=602714
Or you can use the Quantifiler kit that has everything in it
https://products.app...e2&catID=601641
If you'd rather make your own IPC, just browse the literature. There are plenty published.
Can't respond to the second question, sorry.
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.
A. Einstein
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