Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

internal control for genomic DNA


  • Please log in to reply
1 reply to this topic

#1 labscientist

labscientist

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 29 July 2010 - 10:32 PM

Hi all,

I would really appreciate some suggestions of internal controls I can use in my PCR using genomic DNA as a template.
I am working with human blood or marrow samples looking for chromosomal rearrangements. Is it possible to quantify rearrangement wrto a some sort of housekeeping gene when working with gNDA?

Thanks very much

#2 Maddie

Maddie

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 289 posts
2
Neutral

Posted 30 July 2010 - 09:25 AM

For the expensive solutions, ABI sells IPC kits if you don't want to make them.
https://products.app...e2&catID=602714

Or you can use the Quantifiler kit that has everything in it
https://products.app...e2&catID=601641

If you'd rather make your own IPC, just browse the literature. There are plenty published.

Can't respond to the second question, sorry.
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein




Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.