extracting RNA from human adipose tissue. Homogenized in Trizol with a PreCellys homogenizer. Then a standard RNA extraction including a centrifugation step after homogenisation to get the aqueous phase (no fats,proteins...). we also use Glycoblue.
Done it 2 times now on my own. Yesterday it worked perfectly. Had lovely blue pellets and when quantifieng spectrometrically I had high values. today I did the exact same. Same bench, same pipettes, same bag of tubes, same chemicals.... No nice pellets(sometimes something faint blue or something redish) and barely any RNA.
This leaves me really wondering what I have could done wrong, since it worked fine yesterday.
Any suggestions what I could be doing wrong?
Thanks for your help!
Edited by Podge, 29 July 2010 - 06:52 AM.