Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

how to keep stock for fungal strain?


  • Please log in to reply
2 replies to this topic

#1 aspleung

aspleung

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 29 January 2002 - 09:37 PM

I have stains of P. marneffei, C. albcan, A. fumigatus, A. flavus, ...etc.
Could you tell me a method to keep stock for these fungus?

#2 lordgords

lordgords

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 05 February 2002 - 06:54 AM

if you want to maintain stocks you are going to have to grow some overnight cultures up,freeze dry them in open vials , and when freeze dried seal up the vials. store at -20 celcius and they should keep indefinately. fialing this you could always maintain viable colonies by replating on to fresh agar every week, however the risk of contamination is great and the is a possibility that after a few months you have isolated a different organism,hope this is of some help.

#3 nagesh

nagesh

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 17 April 2002 - 09:36 PM

best way is to lyophilize the culture,

try with mineral oil

or else culture once in 15days






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.