Change of colour in cell culture media...?
Posted 29 July 2010 - 01:41 AM
I am using Hams F12 media for my cells, which i make it in house from hams F12 powder. My query is regarding the colour change in my media. i make a couple of bottles evrery time i make some media, which intially has an reddish orange colour. once i start using my media it turns to pure pink in a week but the bottle that i havent yet opened remains the same orange reddish colour...? i was wondering if any body could help me understand the reason behind it. i would also like to add that this change in colour doesn't effect the growth of my cells but i am just curious and thought it would be interesting to know..!!
thank you all in advance.
Posted 29 July 2010 - 03:02 AM
Mammalian cells grow best at roughly neutral pHs and as they grow and produce waste they acidify the media turning it a more orange-yellow colour. I think my media is ok, even if it does shift colour a little. If you see particles/solids in your media it may have become contaminated.
You may also want to make sure you keep your media at 4*C and minimise the amount of time you have to warm it for cell culture and minimise the time the actual media is exposed to the atmosphere. That is all I can think of! I don't think that it's too alarming that the media gets a little pinker...
Posted 29 July 2010 - 02:02 PM