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why my protein precipitates?


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#1 parveen

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Posted 28 July 2010 - 10:46 AM

1) i am trying to crystallize my protein and for that i lyophilized my protein sample. After obtaining in powder form, when i tried to redissolve it in water, it precipitated. Why?

2) I have another batch's protein where i think lyophilization was a bit incomplete in the sense that it still is flaky, this one behaves very transparent upon redissolving. Why?

Can somebody throw a light?

Thanks
Parveen

#2 SUVEAR

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Posted 03 September 2010 - 06:28 AM

Hi,

Maybe during the lyophilization process your protein changed its structure, you can try redisolved in DMSO and water.

1) i am trying to crystallize my protein and for that i lyophilized my protein sample. After obtaining in powder form, when i tried to redissolve it in water, it precipitated. Why?

2) I have another batch's protein where i think lyophilization was a bit incomplete in the sense that it still is flaky, this one behaves very transparent upon redissolving. Why?

Can somebody throw a light?

Thanks
Parveen


Edited by SUVEAR, 03 September 2010 - 06:28 AM.


#3 mdfenko

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Posted 03 September 2010 - 08:22 PM

think "degree of hydration". just like with nucleic acids, some proteins don't like to be dried completely.
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genius does what it must
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