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Lidocaine/EDTA for lifting cells

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#1 anniek29



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Posted 28 July 2010 - 09:25 AM


I currently use a lidocaine/EDTA solution (4mg/ml lidocaine and 10mM EDTA) to lift RAW264.7 macrophages. I do not understand how the lidocaine works- can anyone help me? The only reference I can find in the literature that may deal with the mechanism is: (In Vitro. 1975 Nov-Dec;11(6):379-81.
Use of the local anesthetic lidocaine for cell harvesting and subcultivation. Rabinovitch M, DeStefano MJ), but I can't access the paper.

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