2 replies to this topic

[walruss111]

Hi,

I need to grow E coli at 30 C as I am sharing the incubator with people growing yeast. Do you know how the growth rate of E coli is affected at 30 C? When do the cultures reach stationary phase?

Thanks

[perneseblue]

walruss111, on 28 July 2010 - 07:14 AM, said:

Hi,

I need to grow E coli at 30 C as I am sharing the incubator with people growing yeast. Do you know how the growth rate of E coli is affected at 30 C? When do the cultures reach stationary phase?

Thanks

The growth rate of e coli will be affected. It will grow slower. However final e coli cell density will be higher than a culture grown at a elevated temperature.

As for stationary phase.. well it depends on how large is the culture,  how large is starting culture used to inoculate this culture, and  occasionally what strain of e coli that is being grown, There are a few slow growing commercial e coli strains.

If you are growing a 2ml culture using a single colony as the innoculant, you will probably find that the culture had reach saturation overnight.

[Chimp]

Growing at 30 degrees is no problem at all. yes, they grow slower, but protein expression for purification (over expression) very often works better at 30 degrees or even lower, as inclusion bodies aren´t formed as extensively.