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How to clean agar contamination for colony PCR


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#1 anonymous

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Posted 15 March 2001 - 10:00 PM

For colony PCR, when you pick up colonies, there is some amount of agar in the template, how can I clean it up?

Thank you.

#2 Patrick Laplante

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Posted 29 October 2004 - 09:10 AM

Use glassmilk

#3 rassen

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Posted 31 October 2004 - 12:17 PM

Small amount of agar picked up with the colonies is not a problem for subsquent PCR reaction. Don't worry about it.




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