hi i have 4 genes to study, and used the normal breast tissue (as positive control) total rna to construct standard curve and to check primer efficiency. i want to use delta-delta ct method. however, only 3 out of 4 genes performed well within the range, while the other seem have very low expression in the postivie control samples. If in this case, should i study that low-expressed gene using other source of sample (other than the normal breast tissue)? will it cause any problem when come to discussion of relationship of these four genes in same samples?
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source of sample and qpcr
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