2 replies to this topic

[ocean_sky83]

Hi

Just wondering if I can keep the cDNA at -20 for around 3-4 weeks before I run my real time pcr... does it compromise the result in any way?? I supposed no??

Thanks

[gfischer]

ocean_sky83, on 27 July 2010 - 08:30 PM, said:

Hi

Just wondering if I can keep the cDNA at -20 for around 3-4 weeks before I run my real time pcr... does it compromise the result in any way?? I supposed no??

Thanks

It should be fine.

[biznatch]

We do real-time PCR all the time, and store cDNA at -20 for months, probably over a year for some samples, and have never noticed any negative effects on subsequent PCR or real-time PCR.