generating cell line knockouts
Posted 27 July 2010 - 04:14 PM
I'm thinking about doing a series of experiments in HEK 293 cells and would love to KO my gene of interest to determine the effects on cell viability and was wondering if I can do that just by homologous recombination in HEK 293 cells. Any thoughts/ answers would be greatly appreciated.
Posted 27 July 2010 - 04:16 PM
Posted 27 July 2010 - 05:14 PM
You can definitely do KO in ordinary cell lines. There should be plenty of literature on the web that says how to do it.
You should also read up about the efficient of the cell line for homologous recombination. Some lines are more efficient, some are very very low.
Posted 27 July 2010 - 11:57 PM