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Opinion about LUX primers /D-LUX assay


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5 replies to this topic

#1 Adrian K

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Posted 27 July 2010 - 08:08 AM

Dear All,

Having read about Invitrogen's claim about D-LUX™ Gene Expression Assays, which they claim is the most sensitive, specific, and cost-effective detection method available for qPCR/qRT-PCR,

I was wondering is there anyone had any experience in it and please share your opinions here.

I also noticed that since Invitrogen merge/acquired Applied Biosystem, there wasn't any marketing push about LUX primers, especially in my country. Is it because TaqMan is still superior than LUX or is just for some business agreement. or maybe I'm just too sensitive.

Or is it because there are major flaws in LUX primer design which they discovered/reported (maybe i missed that article)?

Thanks.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

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#2 Maddie

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Posted 02 August 2010 - 08:27 AM

OK Adrian, since nobody responded, I will ;) although my response won't be very useful I'm afraid.
I used LUX in 2004 and they worked fine. I liked the idea of not using a probe like the TaqMan does.
I wouldn't say one is better than the other, the concept is simply different.

6 years later, we are back to Taqman though. Because I am not doing qPCR anymore and my colleague who does got her protocol from another colleague who uses TaqMan.

Attached Thumbnails

  • the LUX.jpg

Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

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#3 Adrian K

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Posted 02 August 2010 - 06:23 PM

OK Adrian, since nobody responded, I will ;) although my response won't be very useful I'm afraid.
I used LUX in 2004 and they worked fine. I liked the idea of not using a probe like the TaqMan does.
I wouldn't say one is better than the other, the concept is simply different.

6 years later, we are back to Taqman though. Because I am not doing qPCR anymore and my colleague who does got her protocol from another colleague who uses TaqMan.


Hi Maddie,
I'm new to real-time PCR, and want to design an assay for end point detection.
Since you had done LUX before, is it possible I get your published paper from you so that I might use it as a reference?
Thanks

Adrian
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#4 Maddie

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Posted 03 August 2010 - 06:54 AM

I didn't publish it, sorry. I found at least one paper I had though. I tried to send it to you by email but it didn't work, so I'm going to attach it here temporarily (I apologize in advance to the moderators). Let me know when you have it and I'll delete the pdf.
Also, you should find more on PubMed.
Good luck.

Edited by Maddie, 03 August 2010 - 09:50 AM.

Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein

#5 Adrian K

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Posted 03 August 2010 - 07:28 AM

Hi MAddie,
I just downloaded it. Thanks again and all the best to you.

Adrian
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#6 Maddie

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Posted 03 August 2010 - 09:50 AM

You're very welcome my dear. :)
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein




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