Adding L-glutamine to DMEM media w/o L-glutamine
Posted 26 July 2010 - 10:22 AM
I'm trying to do an MTT assay using no phenol-red, no L-glutamine DMEM media and will be transferring mesenchymal stem cells from (+)phenol red (+) L glutamine media into this no L-glutamine media, no phenol-red media [b/c phenol red negatively affects MTT results].
I was wondering, how much L-glutamine should I add into the no-L glutamine DMEM? There is 500 ml of media.
Posted 27 July 2010 - 02:22 AM
Posted 27 July 2010 - 04:05 PM
Posted 27 July 2010 - 04:34 PM
Posted 28 July 2010 - 04:10 AM