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Stimulation of transcription


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4 replies to this topic

#1 cellculturenovis

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Posted 26 July 2010 - 04:14 AM

Hi Everyone
I am very new to the cell culture scene so i was wondering if anyone can help me?
I have a T cell line and i want to stimulate transcription of my gene of interest. How do i find out what would stimulate transcription of my gene?
Also can you buy transcription factors? if so where from?

#2 laurequillo

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Posted 26 July 2010 - 05:41 AM

Hi Everyone
I am very new to the cell culture scene so i was wondering if anyone can help me?
I have a T cell line and i want to stimulate transcription of my gene of interest. How do i find out what would stimulate transcription of my gene?
Also can you buy transcription factors? if so where from?


First you have to know where is your gene involved, which pathway...

It could be PMA,Ionomycin,LPS...
"He must be very ignorant for he answers every question he is asked" Voltaire

"This is SPARTA!"

"Im the goddamn batman"

#3 cellculturenovis

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Posted 26 July 2010 - 06:22 AM

The gene is involved in intracellular transport along microtubules. The only pathways i can find are cytoskeleton remodeling and cytoplasmic microtubules.
However i have found some transcription factor binding sites in the promoter pol2, NFkappaB etc.

#4 laurequillo

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Posted 26 July 2010 - 06:25 AM

The gene is involved in intracellular transport along microtubules. The only pathways i can find are cytoskeleton remodeling and cytoplasmic microtubules.
However i have found some transcription factor binding sites in the promoter pol2, NFkappaB etc.


well, so you can use something that activates NfKB, and see what happens
"He must be very ignorant for he answers every question he is asked" Voltaire

"This is SPARTA!"

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#5 cellculturenovis

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Posted 26 July 2010 - 07:18 AM

such as LPS?
Where can i buy purified LPS?




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