5 replies to this topic

[cellculturenovis]

Hi Everyone
I am very new to the cell culture scene so i was wondering if anyone can help me?
I have a T cell line and i want to stimulate transcription of my gene of interest. How do i find out what would stimulate transcription of my gene?
Also can you buy transcription factors? if so where from?

[Rsm]

How about www.google.com? I could also recommend www.yahoo.com.
Type in "T cell line stimulate transcription of (gene of interest)", you'll find lots of pages.
A more scientific approach http://www.ncbi.nlm....ov/sites/gquery or http://scholar.google.co.uk/.

TF you can find here http://mgc.nci.nih.gov/, here http://image.hudsona...ery_tools.shtml or here http://fantom3.gsc.riken.jp/.

hope this helps,

Cheer,

Minna

[laurequillo]

Minna, on 26 July 2010 - 04:29 AM, said:

How about www.google.com? I could also recommend www.yahoo.com.
Type in "T cell line stimulate transcription of (gene of interest)", you'll find lots of pages.
A more scientific approach http://www.ncbi.nlm....ov/sites/gquery or http://scholar.google.co.uk/.

TF you can find here http://mgc.nci.nih.gov/, here http://image.hudsona...ery_tools.shtml or here http://fantom3.gsc.riken.jp/.

hope this helps,

Cheer,

Minna

that page, google.com...is a new one, right?  

Edited by laurequillo, 26 July 2010 - 05:46 AM.

[Rsm]

yeah, sorry, but your question is just a bit too unspecific. So I'd suggest you either search yourself, or you provide us with some more info.
LPS you can get at Sigma.

Cheers,

minna

[cellculturenovis]

Looking at UCSC i have found NFKB and POL2 binding sites what is thought to be the promoter. Could i stimulate transcription by pol2? how?

[cellculturenovis]

Also i have looked at purchasing LPS, would the method used to extract the LPS affect my experiment? eg phenol extraction or trichloroacetic acid extraction?