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Variation accross a plate


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6 replies to this topic

#1 Gradstudent78

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Posted 24 July 2010 - 07:57 AM

How much variation should one expect across a plate for measures of the same sample (using sandwich elisa)? How much would be considered acceptable? Thanks.

#2 sgt4boston

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Posted 26 July 2010 - 02:30 AM

The lowest possible %CV is ideal...</=5% great </=10% good... if you are doing the test manually you may observe an 'end of run' effect...due to the time delay from beginning to end of the assay. Hope this helps you.

#3 Prep!

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Posted 26 July 2010 - 02:58 AM

10% is acceptable universally!!! some people also accept 20% It depends on ur application!!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#4 Gradstudent78

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Posted 31 July 2010 - 03:40 PM

10% is acceptable universally!!! some people also accept 20% It depends on ur application!!!



Thanks, that's pretty much what I was thinking, 10%

#5 Gradstudent78

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Posted 02 August 2010 - 05:21 PM

Right now I'm getting between 10-20% across the plate when examining 10 sets of the same replicates (although the individual pairs are at CV < 10), any tips on things to do to lower this number? Thanks.

#6 sgt4boston

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Posted 12 August 2010 - 06:46 AM

Improving %CV:
1. automated washing
2. check calibration of pipetes
3. make sure the level of coating and reaction are different
4. with manual washing allow wash to sit 10-15 sec before decanting
5. is the number of washes sufficient
6. do you note an end of run effect.....is time critical
7. are you using a multichannel pipettor..minimize time differences

#7 wcw

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Posted 04 January 2011 - 09:29 AM

We use a 15% CV% cutpoint for our within-run duplicate standards.




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