Posted 22 July 2010 - 05:05 PM
Posted 22 July 2010 - 11:17 PM
Posted 23 July 2010 - 12:01 AM
it is not necessary to subtract the blank from all readings... u will still get the same results.... the slope remains the same!!!just that the blank subtraction gives the od or rfu values which are only of the analyte and not contributed by the blank and u know of the LOD and LOQ!!!
i dunno for elisa s (4PL graphs) but it shud hold true for all assays
Edited by Prep!, 23 July 2010 - 12:02 AM.